Abstract: In order to study the effect of acid mine drainage (AMD) from mine closure on the permeability stability of overlying carbonate rocks, acid mine drainage and tap water were used as permeate solution to conduct the tests of seepage-corrosion, 3D laser scanning, ion concentration detection, and scanning electron microscopy (SEM) on single fractured limestone. The results show that, for limestone fracture seepage-corrosion, the solute concentration, permeability and seepage pressure are closely related to confining pressure and solution acidity; the corrosion of the limestone fracture surface shows a "honeycomb" hole, and the matrix shows a "sawtooth" form; with the continuous occurrence of seepage, the fracture width decreases, and the joint roughness coefficients(JRC) decreases; in addition, the corrosion and precipitation materials adhere to the fracture section to form a shell, resulting in the blockage of key seepage channels. The permeability and Ca ²⁺ concentration gradually decrease, and the permeability pressure and total iron concentration increase.The phenomenon of corrosion, precipitation and seepage decline in the process is mainly controlled by the mineral composition, microstructure, acid solubility and hydrodynamic conditions of the medium. The research results can provide some theoretical guidance for mine environmental management and mine rock stability.